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TRENDING OPEN SCIENCE
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Cytosplore-Transcriptomics: a scalable inter-active framework for
single-cell RNA sequenc-ing data analysis
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By
Tamim Abdelaal
Jeroen Eggermont
Thomas Hollt
Ahmed Mahfouz
Marcel Reinders
Boudewijn Lelieveldt
DOI: 10.1101/2020.12.11.421883
On bioRxiv: https://biorxiv.org/content/10.1101/2020.12.11.421883v1
The ever-increasing number of analyzed cells in Single-cell RNA
sequencing (scRNA-seq) experiments imposes several challenges on the
data analysis. Current analysis methods lack scalability to large
datasets hampering interactive visual exploration of the data. We
present Cytosplore-Transcriptomics, a framework to analyze scRNA-seq
data, including data preprocessing, visualization and downstream
analysis. At its core, it uses a hierarchical, manifold preserving
representation of the data that allows the inspection and annotation
of scRNA-seq data at different levels of detail. Consequently,
Cytosplore-Transcriptomics provides interactive analysis of the data
using low-dimensional visualizations that scales to millions of cells.
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